An interesting problem is how activated synapses of a single neuron become selectively modified as a result of synaptic plasticity. It is known, for example, that synaptic modifications can occur selectively at one group of synapses, but not at another group of synapses on the same neuron. This property is known as "input-" or "synapse specificity". It is plausible that the selective degradation of proteins that restrict or limit plasticity may be required for these synaptic changes to occur. Alternatively, various proteolytic activities may provide specificity for long-term synaptic changes. This could be accomplished through the degradation of some proteins at specific locations or by targeting regulatory components of a proteolytic pathway to modified or unmodified sites.
We study the UPS’s role in synaptic plasticity. One approach we take is to use genetically encoded fluorescent-based proteasome (or “degradation”) reporters and time-lapse confocal microscopy to assay how neuronal activity modulates the rate of degradation within the spines and dendrites of neurons. Using these techniques, we hope to understand how and why the UPS is activated at or recruited to synapses in response to neuronal activity.
We are also interested in identifying the protein targets of the UPS at synapses. In particular, we study how the UPS regulates alpha-amino-3-hydroxy-5-methyl-4-isoxaolepropionic acid (AMPA) type ionotropic glutamate receptor trafficking in neurons. The removal and insertion of AMPA receptors at glutamatergic synapses is one mechanism to either decrease or increase synaptic strength. We and others have found that the activity of the UPS is required for agonist-mediated endocytosis of AMPA receptors. The simple model is that protein degradation via the UPS is required for normal AMPA receptor trafficking in neurons. Using molecular and biochemical approaches, we hope to identify the components of the UPS and protein targets involved AMPA receptor trafficking.
We are also interested in neurodegenerative disease. In humans, many neurodegenerative diseases are characterized post-mortem by anatomical hallmarks visible by standard light microscopy. Alzheimer’s and Lewy body disease are the most common causes of dementia in elderly populations. These diseases are characterized by neurofibrillary tangles (NFT), plaques, and lewy bodies (large dense inclusions). Familial Parkinson’s disease is also characterized by Lewy bodies. In addition, large dense inclusions are characteristic of many of the CAG repeat diseases such as Huntington and ALS. Biochemical and immunohistochemical characterization of these inclusions, tangles, and neuritic plaques has indicated that they contain high concentrations of ubiquitin and ubiquitinated proteins. Does the ubiquitin-proteasome pathway contribute to these abnormalities and to neuronal death? We hope that the understanding of UPS in normal neuronal function will help elucidate how it may be involved in neuronal dysfunction and disease.
Djakovic, S.N., Marquez-Lona, E.M., Jakawich, S.K., Chu, C., Sutton, M.S., Patrick, G.N. "Phosphorylation of Rpt6 regulates proteasome function and synaptic strength" (2012) J. Neuroscience in press
Schwarz, L.A., and Patrick, G.N. " Ubiquitin-dependent endocytosis, trafficking and turnover of neuronal membrane protein". (2011) Mol Cell Neurosci Aug 22. [Epub ahead of print]
Schwarz, L.A., Hall, B.J., and Patrick, G.N. "Activity-Dependent Ubiquitination of GluA1 Mediates a Distinct AMPAR Endocytosis and Sorting Pathway". (2010) J. Neuroscience 30(49):16718-29.
Keil, J, Shen, Zhouxin, Briggs, S, and Patrick, G.N. "Regulation of STIM1 and SOCE by the ubiquitin-proteasome system (UPS)" (2010) PLoS One Oct 18;5(10):e13465.
Jakawich S, Nealy R, Djakovic S, Patrick G.N., Sutton M. The Ubiquitin Proteasome System mediates slow homeostatic changes in synaptic strength. (2010) Neuroscience 29;171(4):1016-31.
Djakovic, S.N., Schwarz, L.A., DeMartino, G.N. and Patrick, G.N., "Regulation of the Proteasome by Neuronal Activity and CaMKII" (2009) J. Biological Chemistry 284(39):26655-65.
Kerjan G, Han E, Dube C, Djakovic S, Patrick G.N., Baram T, Heinemann S, Gleeson J. (2009). Mice lacking doublecortin and doublecortin-like kinase 2 display altered hippocampal neuronal maturation and spontaneous seizures. Proc Natl Acad Sci U S A. Apr 21;106(16):6766-71. Epub 2009 Apr 2.
Cartier, A.E., Djakovic, S.N., Wilson, S. and Patrick, G.N. (2009). Regulation of synapse structure by the ubiquitin c-terminal hydrolase UCH-L1. J Neuroscience 29(24):7857-7868.
Djakovic, S.N., Schwarz, L.A., DeMartino, G.N. and Patrick, G.N. (2009). Regulation of the proteasome by neuronal activity and CaMKII. J Biol Chem Aug 7. [Epub ahead of print]
Patrick, G.N. (2006). Synapse formation and plasticity: recent insights from the perspective of the ubiquitin proteasome system. Curr Opin Neurobiol Feb; 16(1):90-4; Jan 18; [Epub ahead of print].
Patrick, G.N., Bingol, B., Weld, H., Schuman, E.M. (2003). Ubiquitin-mediated proteasome activity is required for agonist-induced endocytosis of GluRs. Curr Biol.;13(23):2073-2081.
Zukerberg, L.R.*, Patrick, G.N., Nikolic, M., Humbert, S., Lanier, L.M., Gertler, F.B., Vidal, M., Van Etten, R.A. & Tsai, L.-H-. (2000). Cables links Cdk5 and c-Abl, Facilitating Cdk5 Tyrosine Phosphorylation and Stimulation of Kinase Activity. Neuron 26(3):6336-46.
Patrick, G.N., Zukerberg, L., Nikolic, M., Dikkes, P., de la Monte, S. & Tsai, L.-H. (1999). Conversion of p35 to p25 deregulates cdk5 activity and promotes neurodegeneration. Nature 402(6762): 615-622.
Huang, D., Patrick, G.N., Moffat, J., Tsai, L.-H., & Andrews, B. (1999). Mammalian Cdk5 is a functional homologue of the budding yeast Pho85 cyclin-dependent protein kinase. PNAS 96(25):14445-14450.
Patrick, G.N., Zhou, P., Kwon, Y.T., Howley, P.M. & Tsai, L.-H. (1998). p35, the neuronal specific activator of cdk5, is degraded by the ubiquitin-proteasome pathway. J. Biol. Chem. 273(37): 24057-24064.
Dr. Patrick received his Ph.D. from Harvard University in 1999 after working in the laboratory of Dr. Li-Huei Tsai. He was a Damon Runyon Cancer Research Foundation and a United Negro College Fund/Merck postdoctoral fellow with Dr. Erin Schuman at the California Institute of Technology. Dr. Patrick joined the faculty in 2004.