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Postdoctoral Openings

Autophagy of lipid droplets

Academic Division:

Biological Sciences

Academic Department/Research Unit:

Molecular Biology

Description:

Applications are invited for a postdoctoral position in the group of Taras Nazarko studying mechanisms of lipophagy, the selective autophagy of lipid droplets (LDs). Lipophagy is accomplished by delivery of LDs from the cytosol to the lysosome (or vacuole in yeast). As in other autophagic pathways, the core autophagic machinery forms the autophagic isolation membrane that sequesters the LD from the cytosol. However, how this autophagic membrane recognizes the LD after lipophagy induction is unknown. Also, it is not clear how lipophagy is kept in check the rest of the time. Therefore, lipophagy selectivity and regulation are the key gaps in our understanding of this pathway. A postdoctoral scholar will develop a project in one of these areas. Mechanistic understanding in these areas is critical for the precise control of lipophagy in humans for the prevention and treatment of various lipid accumulation diseases, like atherosclerosis and obesity. Initial appointment is for 1 year, with possible extension for up to 5 years of overall postdoctoral training. Salary is commensurate with experience (NIH NRSA scale).

Successful applicants should:

  1. Have a recent PhD in biochemistry, genetics, molecular or cell biology.
  2. A strong background in either YEAST or ZEBRAFISH genetics, protein biochemistry and fluorescence microscopy. Expertise with mammalian cells is a plus but not essential. Preference will be given to candidates with experience in AUTOPHAGY or LIPID DROPLETS.

How to apply:

Please send your CV, a brief (1-2 paragraphs) statement of research interests and contact information of 3 references to Taras Nazarko, tnazarko@ucsd.edu.

Proteomics and protein homeostasis

Academic Division:

Biological Sciences

Academic Department/Research Unit:

Cell and Developmental Biology

Description:

Applications are invited for a junior or senior postdoctoral position in the laboratory of Eric Bennett in the section of Cell and Developmental Biology at the University of California, San Diego. Applicants with previous postdoc experience are encouraged to apply.

Research in the Bennett lab uses integrative approaches ranging from biochemistry to quantitative proteomics to investigate how the ubiquitin-proteasome system regulates protein homeostasis. Protein homeostasis dysfunction has been implicated in a wide array of aging associated disorders from cancer to neurodegenerative disease.

Projects within the Bennett lab are aimed at uncovering and characterizing novel ubiquitin-dependent regulatory mechanisms that govern the response to protein homeostasis stress. To accomplish this, the lab also develops and utilizes quantitative proteomics methods to interrogate proteome dynamics in response to genetic and pharmacological disruptions of the ubiquitin-proteasome system.

Successful applicants will have the opportunity to interact and work with biotech companies through existing collaborative industry sponsored research projects.

The following are examples of research in the Bennett lab.

The Unfolded Protein Response Triggers Site-Specific Regulatory Ubiquitylation of 40S Ribosomal Proteins.
Higgins R, Gendron JM, Rising L, Mak R, Webb K, Kaiser SE, Zuzow N, Riviere P, Yang B, Fenech E, Tang X, Lindsay SA, Christianson JC, Hampton RY, Wasserman SA, Bennett EJ.
Mol Cell. 2015 Jul 2;59(1):35-49

Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40

Successful applicants should:

  1. Either currently have a Ph.D. degree or will achieve a Ph.D. prior to initiating work
  2. Have demonstrable expertise in at least a subset of the following: mammalian cell culture, protein biochemistry, molecular biology, or quantitative mass spectrometry.
  3. Have experience with mass spectrometry-based proteomics in terms of sample preparation, chromatography, instrument methods, data acquisition and analysis. Preference will be given to applicants with experience in post-translation modifications, in particular ubiquitylation.
  4. Enjoy a dynamic work environment with many opportunities for exciting collaborative research with labs both inside and outside UCSD.

How to apply:

Self-motivated individuals are encouraged to send their CV, a brief (1-2 paragraphs) statement of interest and contact information of two references to Eric Bennett, e1bennett@ucsd.edu.

All positions are contingent on funding becoming available.

Applicants are welcome to include in their cover letters a personal statement summarizing leadership efforts and/or contributions to diversity. UC San Diego is an equal opportunity/affirmative action employer with a strong institutional commitment to the achievement of diversity among its faculty and staff.