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Emily Troemel

Research

How do pathogens attack their hosts, and how do hosts defend themselves against these attacks? We are using the nematode C. elegans to study these questions of host/pathogen interactions in the intestine. The nematode C. elegans provides a wonderful system in which to study these questions. The worm has intestinal cells that are similar in structure to human intestinal cells, but C. elegans is transparent and easy to study in lab. C. elegans lacks a professional immune system, and instead relies solely on epithelial defense to fight off pathogen attack. It is being increasingly appreciated that epithelial cells in the intestine are involved in detecting and responding to pathogens in mammals, and are implicated in inflammatory disorders. It is critical to understand more about how these cells detect and respond to pathogens. What we find in C. elegans intestinal immunity may be directly applicable to host/pathogen interactions in the human intestine.

Microsporidia infecting C. elegans intestine

Microsporidia infecting C. elegans intestine – microsporidia are labeled in red, C. elegans intestine labeled in green, and DNA labeled in blue.

Our research focuses on two different pathogens. The first pathogen is a natural intracellular pathogen isolated from a wild-caught C. elegans strain, which we have found defines a genus and species of microsporidia (intestinal infection of C. elegans intestine shown below). Microsporidia are poorly understood eukaryotic pathogens that are emerging pathogens of humans and can cause problems for immunocompromised patients. The second pathogen is an extracellular bacterial pathogen called Pseudomonas aeruginosa, which is a clinically relevant pathogen that causes opportunistic infections in humans.

Publications

Troemel Lab selected publications:

Research Articles

  • The C. elegans CCAAT-Enhancer-Binding Protein Gamma Is Required for Surveillance Immunity. Reddy KC, Dunbar TL, Nargund AM, Haynes CM, Troemel ER. Cell Rep. 2016 Feb 10. pii: S2211-1247(16)30033-X. doi: 10.1016/j.celrep.2016.01.055. [Epub ahead of print] PMID:26876169
  • Small GTPases promote actin coat formation on microsporidian pathogens traversing the apical membrane of C. elegans intestinal cells. Szumowski SC, Estes KA, Popovich JJ, Botts MR, Sek G, Troemel ER. Cell Microbiol. 2015 Jul 6. doi: 10.1111/cmi.12481. [Epub ahead of print] PMID:26147591
  • Balla KM, Andersen EC, Kruglyak L, Troemel ER. A wild C. elegans strain has enhanced epithelial immunity to a natural microsporidian parasite. PLoS Pathogens. 2015 Feb 13;11(2):e1004583. doi: 10.1371/journal.ppat.1004583. PMID:25680197
  • Szumowski SC, Botts MR, Popovich JJ, Smelkinson MG, Troemel ER. The small GTPase RAB-11 directs polarized exocytosis of the intracellular pathogen N. parisii for fecal-oral transmission from C. elegans. Proc Natl Acad Sci U S A. 2014 May 19. PMCID: PMC4050618
  • Bakowski MA, Desjardins CA, Smelkinson MG, Dunbar TA, Lopez-Moyado IF, Rifkin SA, Cuomo CA, Troemel ER. Ubiquitin-Mediated Response to Microsporidia and Virus Infection in C. elegans. PLoS Pathog. 2014 Jun 19;10(6):e1004200. PMCID: PMC4063957
  • Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, Didier ES, Fan L, Heiman DI, Levin JZ, Young S, Zeng Q, Troemel ER. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. Genome Research 2012 Dec;22(12):2478-88. PMCID: PMC3514677
  • Dunbar TL, Yan Z, Balla KM, Smelkinson MG, Troemel ER. C. elegans Detects Pathogen-Induced Translational Inhibition to Activate Immune Signaling. Cell Host Microbe. 2012 Apr 19;11(4):375-86. PMCID: PMC3334869

Reviews

  • The Development of Genetic Modification Techniques in Intracellular Parasites and Potential Applications to Microsporidia. Reinke AW, Troemel ER. PLoS Pathog. 2015 Dec 31;11(12):e1005283. doi: 10.1371/journal.ppat.1005283. eCollection 2015 Dec. No abstract available. PMID:26720003
  • Szumowski SC, Troemel ER. Microsporidia-host interactions. Curr Opin Microbiol. 2015 Apr 3;26:10-16. doi: 10.1016/j.mib.2015.03.006. [Epub ahead of print] Review. PMCID: PMC4577307
  • Cohen LB, Troemel ER. Microbial pathogenesis and host defense in the nematode C. elegans. Curr Opin Microbiol. Curr Opin Microbiol. 2015 Feb;23:94-101. PMCID: PMC4324121

Biography

Emily Troemel received her B.S. from the University of Wisconsin-Madison and then spent a year in Japan before receiving her Ph.D. in Cell Biology at UC-San Francisco. She worked for a start-up biotech company in the Bay Area before returning to academia to do a postdoc at Massachusetts General Hospital and then joined the UCSD faculty in 2008. Since joining UCSD, she has received a Searle Scholars Award, David & Lucile Packard Foundation Award and a Burroughs Wellcome Fund Investigators in the Pathogenesis of Infectious Disease Award.